GRANT
NUMBER:
NA66FD0107
NMFS NUMBER: 95-NWR-004
REPORT
TITLE:
Microencapsulated Delivery of Amino Acids to Striped Bass
and other Altricial Larvae
AUTHOR:
Chris
Langdon
PUBLISH
DATE:
June 18, 1998
AVAILABLE
FROM:
National Marine Fisheries Service, Northwest Region, Attn:
Kevin Ford, 7600 Sand Point Way NE, BIN C15700, Bldg #1,
Seattle, Washington 98115. TELEPHONE: (206)
526-6115
ABSTRACT

The purpose
of the project was to develop and evaluate a microencapsulation
method for delivering water-soluble amino acids to larvae
of stripped bass and other altricial fish species. Due
to difficulties in obtaining stripped bass larvae throughout
the duration of the project, most of the work was conducted
with zebrafish larvae which served as a model fish species.
Using phenol red as convenient substitute for amino acids,
the lipid-composition of LWM was modified to maximize
delivery efficiency. Spray techniques were developed for
large-scale (100 gram amounts) production of LWM and lipid
microspheres (spray beads). Unfortunately, leakage rates
of amino acids from LWM were significantly greater than
for phenol red and about 80% of encapsulated glycine was
lost from LWM within 1 hr suspension in water. Attempts
to reduce amino acid leakage rates unsuccessful. Spray
techniques were successfully developed for a range of
microgel particles for delivery of LWM to larvae. Feeding
and growth experiments with zebrafish larvae indicated
that AGP had good potential for delivering artificial
diets to larvae.