SALTONSTALL KENNEDY GRANT PROGRAM
Microencapsulated Delivery of Amino Acids to....
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GRANT NUMBER:  NA66FD0107           NMFS NUMBER:  95-NWR-004

REPORT TITLE:  Microencapsulated Delivery of Amino Acids to Striped Bass and other Altricial Larvae

AUTHOR:  Chris Langdon

PUBLISH DATE:  June 18, 1998

AVAILABLE FROM:  National Marine Fisheries Service, Northwest Region, Attn: Kevin Ford, 7600 Sand Point Way NE, BIN C15700, Bldg #1, Seattle, Washington 98115.  TELEPHONE:  (206) 526-6115

ABSTRACT

The purpose of the project was to develop and evaluate a microencapsulation method for delivering water-soluble amino acids to larvae of stripped bass and other altricial fish species. Due to difficulties in obtaining stripped bass larvae throughout the duration of the project, most of the work was conducted with zebrafish larvae which served as a model fish species. Using phenol red as convenient substitute for amino acids, the lipid-composition of LWM was modified to maximize delivery efficiency. Spray techniques were developed for large-scale (100 gram amounts) production of LWM and lipid microspheres (spray beads). Unfortunately, leakage rates of amino acids from LWM were significantly greater than for phenol red and about 80% of encapsulated glycine was lost from LWM within 1 hr suspension in water. Attempts to reduce amino acid leakage rates unsuccessful. Spray techniques were successfully developed for a range of microgel particles for delivery of LWM to larvae. Feeding and growth experiments with zebrafish larvae indicated that AGP had good potential for delivering artificial diets to larvae.


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