and Derivative Approaches toward Marine Biotoxins Standards
National Marine Fisheries Service, Southeast Region, 9721
Executive Center Drive, North Koger Building, St. Petersburg,
FL 33702. PHONE: (813) 570-5324
The goal of
this project was the mass cultivation of marine dinoflagellates
responsible for neurotoxic shellfish poisoning, paralytic
shellfish poisoning, and diarrhetic shellfish poisoning.
Laboratory cultures of Ptychodiscus brevis, Gymnodinium
catenatu, and Prorcentrum lima were quantified
and purified to homogeneity. Saxitoxin and its
derivatives and the brevetoxins and derivatives were the
focus of this work. Twenty-five derivatives and
potential metabolites of the brevetoxins were produced
and the lethality of each was evaluated. Purification
was achieved using HPLC under reverse phase elution conditions.
Saxitoxin and its derivatives were produced from G.
catenatum cultures and were quantified by HPLC using
the Sullivan post-column fluorescent derivatization method,
and by capillary electrophoresis. Synthetic saxitoxin
protocols were thoroughly investigated and were found
to be unprofitable and unattainable for producing saxitoxin
in large amounts for the industry as standards.