GRANT
NUMBER:
NA47FD0296
NMFS NUMBER: 93-SER-034
REPORT
TITLE:
Reproduction of Flounder: Biotechnology for Controlled
Breeding in Fishery Enhancement and Aquaculture
AUTHOR:
Sullivan, Dr. Craig V.
PUBLISH
DATE:
November
20, 1997
AVAILABLE
FROM:
National Marine Fisheries Service, Southeast Region, 9721
Executive Center Drive, North Koger Bldg., St. Petersburg,
FL 33702. PHONE: (813) 570-5324
ABSTRACT
The goal of
the project was to develop the techniques and knowledge
required to establish the routine and controlled breeding
of flounder for fishery enhancement and aquaculture.
The specific objectives were to: (1) acquire the
practical knowledge of reproduction necessary for advances
in controlled breeding, (2) found captive broodstocks
using reconditioned wild adults, (3) develop a controlled
photothermal conditioning regime effective for stimulating
gonadal growth and maturation, (4) induce spawning with
mammalian gonadotropin hormone releasing hormone analogue
(mGnRHa) chronically released from implanted pellets,
and (5) create 'clinical' non-destructive assays of maturation
based on circulating levels of reproductive hormones and
proteins.
