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GRANT NUMBER:  NA37FD0144           NMFS NUMBER: 92-NWR-008

REPORT TITLE:  Physical, Chemical, and Genetic Differences Associated with Harmful Marine Phytoplankton:  Heterosigma and Domoic Acid Producers

AUTHOR:  Banse, K.; Cattolico, R.A.; and Taub, F.B.

PUBLISH DATE:  May 17, 1994

AVAILABLE FROM:  National Marine Fisheries Service, Northwest Region, 7600 Sand Point Way, NE, BIN C15700, Bldg. 1, Seattle, WA 98115.   PHONE: (206) 526-6117

ABSTRACT

The objectives of this project were: to define the nutrient and physical conditions that cause Heterosigma to be toxic to certain fish; develop a chemi-luminescent probe for the rapid identification; and, survey the regional and seasonal distribution of Pseudonitzschia, a planktonic diatom that produces domoic acid.  Researchers found that decreased surface salinity causes Heterosigma to become toxic, or the mode of toxicity, were not evident.  Development of a fluorescent detection method for Heterosigma was initiated.  A methodology was developed to induce toxicity for Heterosigma.   Water samples were collected and examined for the presence of Pseudonitzschia and other harmful species. Lists of all phytoplankton species found were enumerated in an attempt to understand more about their spatial and temporal distribution and abundance in western Washington marine waters. Thirteen cultures of Pseudonitzschia were isolated from samples taken during the project.

 
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