KENNEDY GRANT PROGRAM
Assessment of Public Health....
| NEWS | BIBLIOGRAPHY
| FAQ | CONTACTS
| LINKS | SEARCH
NMFS NUMBER: 96-SER-073
TITLE: Molecular Assessment of Public Health
Suitability of Shellfish for Human Consumption
FROM: National Marine Fisheries Service,
Southeast Region, 9721 Executive Center Drive, St Petersburg,
FL 33702. TELEPHONE: (727) 570-5324
in human fecal pollution that are carried by shellfish
can lead to serious health hazards. Existing sanitation
standards rely on the detection of bacteria, not viruses,
present in feces. Norwalk-type viruses (NLV) and
hepatitis A virus (HAV) have been the most common viral
shellfish-associated disease agents. The goals of
this research were to develop an invective assay for NLV
and other caliciviruses and to develop an immunocapture
reverse-transcriptase polymerase chain reaction (RT-PCR)
assay for the detection of these viruses in shellfish.
Approaches explored for the invectivity assay included
evaluation of primary and continuous cell lines, of different
additives and physical conditions, and of different methods
of virus inoculum preparation. None of these approaches
provided consistent evidence of virus replication.
However, an immunocapture RT-PCR assay for the detection
of Norwalk virus was developed along with modifications
of the RT-PCR assay to improve its performance characteristics.
The new assay allows the detection of Norwalk virus added
to shellfish tissues.
Statement | Disclaimer
Fisheries, 1315 East West Highway, SSMC3, Silver Spring, MD