Inter-Laboratory Investigation on the Feasibility....
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GRANT NUMBER:  NA86FD0110          NMFS NUMBER:  98-NER-037

REPORT TITLE: Inter-Laboratory Investigation on the Feasibility of Otolith Microconstituent Analysis to Characterize Atlantic Bluefin Tuna Structure

AUTHOR: David H. Secor

PUBLISH DATE:  March 16, 2001

AVAILABLE FROM:  National Marine Fisheries Service, Northeast Region, One Blackburn Drive, Gloucester, MA 01930-2298.  PHONE: (978) 281-9267


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Protocols used to collect and prepare otoliths for chemical analysis may result in either contamination or loss of elements, thus biasing population studies in unknown ways. We evaluated precision and bias associated with collection and cleaning procedures for three Atlantic tuna species: Atlantic bluefin tuna (Thunnus thynnus), yellowfin tuna (T.albacares) and blackfin tuna (T.atlanticus). Elemental concentrations were measured using solution-based inductively coupled plasma mass spectromentry (ICPMS) and atomic absorption spectrophotometry (AAS). Seven elements were present above detection limits in all samples (Na, Mg, K, Ca, Mn, Sr, Ba). Mean concentrations of all seven elements were statistically indistinguishable in fresh pairs of otoliths of T. thynnus ( mean error 5%, range 2-8%) and T. albacares (mean error 5%, range 3-7%); no indication of a left versus right otolith effect was observed. Otolith elemental concentrations were size-dependent, and significant inverse relationships were observed for Mg, Na, and K. Deliberate contamination of previously cleaned samples using a 10-ppm solution of a mixture of elements demonstrated that otoliths easily acquire surface contamination. Recleaning contaminated otoliths restored elemental concentrations to levels similar to control otoliths. Precision of paired comparisons between cleaned otoliths and those exposed to contamination and recleaned was high (mean error 6%). The effects of storage at two temperatures (7 d at -20 C; 3 d at 1 C) were investigated. For K, Ca, Sr, Mn, and Ba, variation between control (removed immediately) and treatment otoliths (in situ freezing or chilling) was similar to variation observed within fresh otolith pairs (mean error: fresh vs. frozen 5% iced 5%).

Statistically significant but small (<10%) post-mortem storage effects were observed for Na and Mg. Estimates of error indexed to natural ranges in otolith chemistry of T. thynnus and T. albacares from different geographic regions in the Atlantic and Pacific showed that error values of several elements (Mg, Ba, Na, K) accounted for a small proportion of the natural range, suggesting levels of precision achieved in this study are suitable for the purpose of stock delineation.

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