Broodstock Selection and Hatchery Development....
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GRANT NUMBER:  NA66FD0045          NMFS NUMBER:  95-AKR-021

REPORT TITLE:  Broodstock Selection and Hatchery Development of Purple-Hinged Rock Scallops (Crassodoma gigantea) for Marine Aquaculture

AUTHOR:  Jon Agosti

PUBLISH DATE:  February 4, 2001

AVAILABLE FROM:  National Marine Fisheries Service, Alaska Region, P.O. Box 21668, Juneau, AK 99802-1668.  TELEPHONE:  (907) 586-7273


After obtaining acquisition and transport permits, 80 adult rock scallops were collected by scuba divers at a southeastern Alaska site, 500 miles from the Qutekcak Shellfish Hatchery. The first 30 scallops were sacrificed for analysis by the Alaska Department of Fish and Game Pathology laboratory and revealed no organisms of transport significance. The hatchery was permitted a maximum of 50 adults for broodstock. Broodstock were spawned and reconditioned regularly throughout the year. Conditioning and larval rearing methods evolved and are described in detail in the final report for this project. Initially, poor larval growth and survival required development of an appropriate diet for larvae and broodstock. Four species of microalgae–Tahitian Isochrysis galbana (tIso), Chaetoceros calcitrans (Ccal), Pavlova lutheri (Pl), and Chroomonas salina (Chsa) were fed at specific cell ratios and increasing density. This diet provided (1) the essential PUFA 22:6n3 at a 22:6n3/20:5n3 ratio greater than two and some 20:4n6; (2) the three sterols: cholesterol, stigmasterol, and -sitosterol; and (3) carbohydrates for incorporation in the gametes. Rock scallop larvae grow about three microns/day at 14 C with about 25 percent survival to the eyed pediveliger stage. The project period ended before adequate development of setting and spat rearing methods were complete.

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