NMFS NUMBER: 95-WO-007
Taura Syndrome of Maine Penaeid Shrimp: Development and
Application of Molecular Detection Methods of TSV from
Domestic Shrimp Aquaculture and Evaluation of Challenge
Studies in Gulf of Mexico Species
Lightner, Dr. Donald V.
December 12, 1996
National Marine Fisheries Service, Southwest Region Science
Center, Galveston Laboratory, 4700 Avenue U, Galveston,
TX 77551. PHONE: (409) 766-3516
Virus (TSV) was the topic of this 1-year research project.
New knowledge on the biology and host range of TSV was
developed as a result of the research effort. The virus
was characterized and tentatively placed in the family
Picornaviridae. Modern detection methods
for the virus were developed, including serological methods
using fluorescent and enzyme-linked detection methods
with polyclonal and monoclonal antibodies to TSV antigens.
Nonradioactive cDNA probes were developed and applied
to in situ hybridization assays for TSV in histological
sections. A PCR method was developed for TSV and successfully
applied to the detection of the virus in fresh hemolymph
samples. Native shrimp species were challenged with TSV.
The white shrimp, Penaeus setiferus, was found
to be highly susceptible to infection and disease in the
postlarval stages, while juveniles of the species were
found to be relatively resistant. In contrast, postlarval
and juvenile stages of the brown shrimp, P. aztecus,
and the pink shrimp, P. duorarum, were found
to be refractory to TSV.