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Taura Syndrome of Maine Penaeid Shrimp:...
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GRANT NUMBER:  NA56FD0621           NMFS NUMBER:  95-WO-007

REPORT TITLE:  Taura Syndrome of Maine Penaeid Shrimp: Development and Application of Molecular Detection Methods of TSV from Domestic Shrimp Aquaculture and Evaluation of Challenge Studies in Gulf of Mexico Species

AUTHOR:  Lightner, Dr. Donald V.

PUBLISH DATE:  December 12, 1996

AVAILABLE FROM:  National Marine Fisheries Service, Southwest Region Science Center, Galveston Laboratory, 4700 Avenue U, Galveston, TX 77551.   PHONE: (409) 766-3516

ABSTRACT

Taura Syndrome Virus (TSV) was the topic of this 1-year research project. New knowledge on the biology and host range of TSV was developed as a result of the research effort. The virus was characterized and tentatively placed in the family Picornaviridae.   Modern detection methods for the virus were developed, including serological methods using fluorescent and enzyme-linked detection methods with polyclonal and monoclonal antibodies to TSV antigens.  Nonradioactive cDNA probes were developed and applied to in situ hybridization assays for TSV in histological sections. A PCR method was developed for TSV and successfully applied to the detection of the virus in fresh hemolymph samples. Native shrimp species were challenged with TSV. The white shrimp, Penaeus setiferus, was found to be highly susceptible to infection and disease in the postlarval stages, while juveniles of the species were found to be relatively resistant. In contrast, postlarval and juvenile stages of the brown shrimp, P. aztecus, and the pink shrimp, P. duorarum, were found to be refractory to TSV.

 
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