NMFS NUMBER: 93-WO-007
Detection and Quantification of Toxic Dinoflagellate Species
Using Molecular Probes
Anderson, Donald M.
February 28, 1997
Marine Fisheries Service, Johnson Laboratory, 219 Fort
Johnson Road, Charleston, SC 29412. PHONE: (803)
of the project was to develop species-specific or strain-specific
assays for harmful algal species. Polyclonal antibodies
(PAbs) to Alexandrium tamarense were produced
and were then affinity purified to minimize cross-reactions.
The initial production of PAbs was highly successful.
Affinity purification required two different approaches,
i.e., positive selection of the anti-Alexandrium
antibodies and negative adsorption with subsequent removal
of the cross-reacting antibodies. The negative adsorption
method appeared to be the best approach; however, even
after adsorption, significant cross-reactions with Pyrodinium
remained. Therefore, negative adsorption proved to be
as challenging and unfruitful as the positive selection
methods for purified antibodies. Despite considerable
effort, the project did not result in a usable, purified
polyclonal antibody. It is now clear that monoclonal
antibodies offer the best chance of producing highly specific
antibodies to Alexandrium.